Decomposition of Atomic Force Microscopy Images Using the Scaling Index Method for the Investigation of DNA Strands
نویسندگان
چکیده
The scaling index method was used in order to analyze atomic force microscopic images of double stranded DNA (plasmid PUC 8) adsorbed to mica. With this algorithm the image can be decomposed into constituents of different dimensionality, i.e. point-like, thread-like, and area-like structures, and residual noise. Special focus is on the evaluation of this method for the future automated analysis of binding events of DNA-specific proteins.
منابع مشابه
Scaling-index method as an image processing tool in scanning-probe microscopy.
The scaling-index method (SIM) is a novel tool for image processing in scanning-probe microscopy. Originating from the theory of complex systems, the SIM can be used in order to extract structural information from arbitrary data sets. This method can readily be applied to the analysis of digital atomic-force microscopy (AFM) images. Especially for biomedical diagnostics, where genetic material ...
متن کاملMagnetic force microscopy using fabricated cobalt-coated carbon nanotubes probes
Magnetic force microscope ( MFM ) is a powerful technique for mapping the magnetic force gradient above the sample surface. Herein, single-wall carbon nanotubes (SWCNT) were used to fabricate MFM probe by dielectrophoresis method which is a reproducible and cost-effective technique. The effect of induced voltage on the deposition manner of carbon nanotubes (CNT) on the atomic force microscope (...
متن کاملHigh Resolution Image with Multi-wall Carbon Nanotube Atomic Force Microscopy Tip (RESEARCH NOTE)
In this paper, a simple and reproducible approach for attaching the multi-wall carbon nanotubes (MWNTs) to the apex of the atomic force microscope probe has been proposed. For this purpose, the dielectrophoresis method was applied due to its simple performance, cheapness and reliability. In this method, various parameters such as voltage, frequency, concentration of carbon nanotubes solution an...
متن کاملInvestigation of Physical Properties of e-Beam Evaporated CdTe Thin Films for Photovoltaic Application
CdTe thin films with 2.8 µm thickness were deposited by electron beam evaporation method. X-ray diffraction, scanning electron microscopy, UV-Vis-NIR spectroscopy and atomic force microscopy (AFM) were used to characterize the films. The results of AFM analysis revealed that the CdTe films have uniform surface. CdTe thin films were heat-treated by SnCl2 solution. Structural analysis using XRD s...
متن کاملDirect DNA Immobilization onto a Carbon Nanotube Modified Electrode: Study on the Influence of pH and Ionic Strength
Over the past years, DNA biosensors have been developed to analyze DNA interaction and damage that have important applications in biotechnological researches. The immobilization of DNA onto a substrate is one key step for construction of DNA electrochemical biosensors. In this report, a direct approach has been described for immobilization of single strand DNA onto carboxylic acid-functionalize...
متن کامل